FIGURE 7.
T−4128 SNP decreases reporter gene expression. Expression of the YY1 and non-YY1 MYO7A promoter constructs in two different human cell types is shown. 100% activity in each cell line was arbitrarily set to the normalized luciferase activity from the non-YY1 C−4128 construct. A, in the ARPE-19 cell line the construct containing the T−4128 SNP, which creates the YY1 binding site, decreases luciferase expression by 41% compared with the construct containing the C−4128 SNP (p = 0.036). B, in the HeLa cell line the construct containing the T−4128 SNP decreases luciferase expression by 46% compared with the construct containing the C−4128 SNP (p = 0.008).