FIGURE 4.

EDD overexpression inhibits phosphorylation of p53 at Ser¹⁵ after DNA damage. A, HEK293 cells were transfected by electroporation with EDD plasmid or empty vector. Two days later, cells were either left untreated or treated by NCS for 1.5 h. Cell lysates were subjected to Western blotting analysis with the indicated antibodies. The FLAG-tagged EDD was immunoprecipitated (IP) with anti-FLAG beads and immunoblotted (IB) with EDD antibody (lower panel). B, HCT116 cells were transfected by electroporation with EDD plasmid or empty vector. Two days later, cells were either left untreated or treated by NCS for 2 h. Cell lysates were subjected to Western blotting analysis. PCNA, proliferating cell nuclear antigen.