FIGURE 9.
EDD siRNA induces G1/S arrest through p53. HCT116 p53+/+ or p53−/− cells were infected with Ad-siGFP or Ad-siEDD#3 at a multiplicity of infection of 600. Forty-eight hours later, cells were labeled with BrdU for 8 h, and the incorporated BrdU was detected with fluorescein isothiocyanate-conjugated anti-BrdU antibody. Upper panels are representative images of 200× magnification. In the left lower panel, the graphs represent means ± standard errors. At least 300 nuclei were scored on each sample for BrdU incorporation by microscope. The experiments were repeated three times. The p values are based on a paired two-tailed t test. Right lower panels, immunoblots of the lysates from this experiment.