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. 2011 Mar 5;286(17):15413–15427. doi: 10.1074/jbc.M111.225946

FIGURE 6.

FIGURE 6.

Effect of iron or NAC supplementation on the DFO- or Dp44mT-induced phosphorylation of JNK and p38. The expression of JNK (phospho-Thr-183/Tyr-185) (A) and p38 (phospho-Thr-180/Tyr-182) (B) after DMS-53 cells were incubated (i.e. first incubation) with either DFO (250 μm) or Dp44mT (25 μm) for 24 h/37 °C and then re-incubated (i.e. second incubation) with either ferric ammonium citrate (100 μg/ml), DFO (250 μm), or Dp44mT (25 μm) for another 24 h/37 °C. The expressions of JNK (phospho-Thr-183/Tyr-185) (C) and p38 (phospho-Thr-180/Tyr-182) (D) after DMS-53 cells were incubated the same way as in A but using NAC (5 mm) in the second incubation rather than FAC. Western blotting was performed using anti-rabbit phospho- and nonphospho-specific antibodies for JNK or p38. Densitometry of phospho- and nonphospho-JNK includes both bands. The panels and the densitometric analysis are either a typical experiment from three performed or the mean ± S.E. of at least three experiments, respectively. **, p < 0.01.