TABLE 3.
Alteration of protein phosphorylation in the MAPK signaling pathway due to DFO or Dp44mT
| Molecule | Phospho-site | Ratioa | Effects of phosphorylation on biological processes (phospho-site) |
|---|---|---|---|
| Increase in phosphorylation due to DFO | |||
| ATF2 | Thr-71 or -53 | 1.14 | Regulates cell cycle, cell growth, and transcription |
| c-Kit | Tyr-721 | 1.16 | Regulates cell adhesion |
| FAK | Tyr-925 | 1.12 | Regulates apoptosis, cell differentiation, and transcription |
| Histone H3.1 | Ser-10 | 1.18 | Unknown |
| HSP27 | Ser-78 | 1.12 | Cytoskeletal reorganization, regulates apoptosis, and cell cycle |
| HSP27 | Ser-82 | 1.16 | Cytoskeletal reorganization and regulates apoptosis |
| HSP27 | Ser-15 | 1.19 | Cytoskeletal reorganization and regulates apoptosis |
| IRS-1 | Ser-312 | 1.12 | Unknown |
| p38 MAPK | Tyr-182 | 1.23 | Cytoskeletal reorganization, regulates apoptosis, cell adhesion, cell cycle, motility; dual phosphorylation on Thr-180 and Tyr-182, which activates the enzyme |
| p53 | Ser-315 | 1.10 | Regulates apoptosis, cell cycle, cell growth, and transcription |
| p53 | Ser-37 | 1.11 | Regulates apoptosis and transcription |
| p53 | Ser-33 | 1.12 | Regulates apoptosis, cell growth, and transcription |
| p53 | Ser-46 | 1.13 | Regulates apoptosis, cell growth, and transcription |
| p53 | Ser-15 | 1.14 | Regulates apoptosis, cell cycle, cell growth, and transcription |
| p53 | Thr-18 | 1.15 | Regulates transcription |
| p53 | Ser-9 | 1.15 | Regulates apoptosis, cell cycle, and transcription |
| SAPK/JNK | Thr-183 | 1.17 | Regulates apoptosis, cell adhesion, and transcription; dual phosphorylation on Thr-183 and Tyr-185, which activates the enzyme |
| SAPK/JNK | Tyr-185 | 1.20 | Regulates apoptosis, cell adhesion, and transcription; dual phosphorylation on Thr-183 and Tyr-185, which activates the enzyme |
| Tau | Ser-356 | 1.14 | Unknown |
| Increase in phosphorylation due to Dp44mT | |||
| Histone H3.1 | Ser-10 | 1.18 | Unknown |
| HSP27 | Ser-15 | 1.10 | Cytoskeletal reorganization and regulates apoptosis |
| HSP27 | Ser-78 | 1.10 | Cytoskeletal reorganization, regulates apoptosis, and cell cycle |
| HSP27 | Ser-82 | 1.15 | Cytoskeletal reorganization and regulates apoptosis |
| p38 MAPK | Tyr-182 | 1.12 | Cytoskeletal reorganization, regulates apoptosis, cell adhesion, cell cycle, and cell motility; dual phosphorylation on Thr-180 and Tyr-182, which activates the enzyme |
| p53 | Ser-37 | 1.10 | Regulates apoptosis, cell growth, and transcription |
| p53 | Thr-18 | 1.11 | Regulates transcription |
| p53 | Ser-15 | 1.12 | Regulates apoptosis, cell cycle, cell growth, and transcription |
| p53 | Ser-9 | 1.13 | Regulates apoptosis, cell growth, and transcription |
| SAPK/JNK | Thr-183 | 1.14 | Regulates apoptosis, cell adhesion, transcription; dual phosphorylation on Thr-183 and Tyr-185, which activates the enzyme |
| SAPK/JNK | Tyr-185 | 1.26 | Regulates apoptosis, cell adhesion, transcription; dual phosphorylation on Thr-183 and Tyr-185; which activates the enzyme |
| Tau | Ser-396 | 1.14 | Unknown |
a Data are relative to control.