Fig. 4.
The effect of hydroxylamine on the proteins in A. subfuscus glue. (A) The left two lanes show the Coomassie-Blue-stained blot, and the right two lanes show the DNPH immunostained duplicate blot. For each pair, the left lane is untreated and the right lane is treated with hydroxylamine (NH2OH). Oxidized BSA controls treated in the same way are shown at the bottom. The numbers on the left show relative molecular mass (×103). (B) Amount of soluble protein in hydroxylamine-treated samples relative to untreated controls for selected proteins. (C) Extent of oxidation of selected proteins from hydroxylamine-treated samples relative to their untreated counterpart. Note that asmp-114 is not included in this graph because its peak could not be easily distinguished from the strong signal for asmp-165. The dashed lines in B and C correspond to the y-value if there was no difference between the control and treatment. Values are means ± s.e.m. (N=5).