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. 2011 May;22(5):902–913. doi: 10.1681/ASN.2010070705

Figure 1.

Figure 1.

Physiologic changes in proximal tubule-specific autophagy-deficient mice. (A and B) Western blot analysis confirmed Atg5 deficiency, suppression of the conversion of LC3-I to LC3-II, and accumulation of p62 in (A) kidney cortexes of 8-week-old Atg5flox/flox; KAP-Cre+ (F/F; KAP) mice (n = 5) and in (B) lysates of isolated proximal tubular cells of 3-week-old Atg5flox/flox; KAP-Cre+ mice (n = 3 to 4). A representative immunoblot is shown. The graph shows the ratios of band intensities of Atg5-Atg12, LC3-II, and p62 to those of actin, standardized to the mean of the control. Megalin was blotted as a marker of the proximal tubular cells. (C) Glycosiuria was observed in 6-month-old Atg5flox/flox; KAP-Cre+ mice (n = 5 to 6). (D) Mild amino aciduria was observed in 6-month-old Atg5flox/flox; KAP-Cre+ mice (n = 5 to 6). Cysteine and serine were not detected. Data are presented as means ± SD. *P < 0.05 versus Atg5flox/flox; KAP-Cre− mice of same age.