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. 2011 May;22(5):902–913. doi: 10.1681/ASN.2010070705

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Copyright © 2011 by the American Society of Nephrology

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Figure 4. — Formation of LC3 dots in kidney proximal tubules of LC3-GFP transgenic mice in response to I/R injury. (A) The presence of LC3-positive dots in the proximal tubules of the kidney of LC3-GFP transgenic mice was examined by immunofluorescence analysis after a sham operation and after 3, 6, 12, 24, 36, and 48 hours after unilateral I/R injury. The number of LC3 dots increased time-dependently after injury for up to 24 hours, after which it decreased. Lotus tetragonolobus lectin (LTA) was used as a marker of proximal tubules, and DAPI staining was performed as a counterstaining. (B and C) LC3 dots colocalized with p62 (B) and ubiquitin dots (C) after I/R injury. Kidney tissues 12 hours after unilateral I/R injury is shown. The figure is representative of a multiple experiments (n = 3 to 5). Bars, 10 (A) and 5 μm (B and C). Magnification, ×352 (A), ×1058 (B), and ×1408 (C).