Table 1.
Differentially expressed proteins in GBM samples identified by MALDI mass spectrometry after DIGE analysis*
| Average ratio GBM/NT(a) | T-test (b) | Protein name | Species | Mt Th.( c) | PI Th. (d) | Accession No. (gi) (e) | Matched Peptides (f) | Coverage % (g) | Score(h) |
|---|---|---|---|---|---|---|---|---|---|
| 7.33 | 0,0060 | hemoglobin | Homo Sapiens | 15834 | 6.76 | 61679606 | 11/82 | 80 | 100 |
| 6.52 | 0.0065 | dihydropyrimidinase -related protein 2 | Homo Sapiens | 62711 | 5.95 | 4503377 | 24/73 | 55 | 225 |
| 6.49 | 0,0058 | apolipoprotein A-1 | Homo Sapiens | 28061 | 5.27 | 90108666 | 17/69 | 50 | 153 |
| 6.47 | 0,035 | human serum albumin | Homo Sapiens | 67174 | 5.57 | 55669910 | 26/59 | 54 | 263 |
| 6.19 | 0,0078 | alpha-2-globin | Homo Sapiens | 15174 | 8.73 | 1335076 | 9/55 | 69 | 86 |
| 5.88 | 0.024 | tubulin beta 2 | Homo Sapiens | 50274 | 4.78 | 4507729 | 22/73 | 43 | 207 |
| 5.83 | 0,0015 | ubiquitin carboxyl-terminal hydrolase isozyme L1 | Homo Sapiens | 25151 | 5.33 | 21361091 | 10/40 | 59 | 131 |
| 5.83 | 0,0022 | ER-60 protease | Homo Sapiens | 57160 | 5.98 | 1208427 | 17/40 | 34 | 170 |
| 4.60 | 0,0085 | MTHSP75 (GRP-75) | Homo Sapiens | 74019 | 5.9 | 292059 | 26/72 | 49 | 208 |
| 4.29 | 0.020 | dihydropyrimidinase-related protein 3 | Homo Sapiens | 62323 | 6.11 | 4503379 | 12/29 | 27 | 76 |
| 4.26 | 0,048 | vimentin variant | Homo Sapiens | 53708 | 5.06 | 62896523 | 25/100 | 59 | 172 |
| higly similar to glial fibrillary acid protein (GFAP) | Homo Sapiens | 49533 | 5.84 | 34536332 | 26/100 | 63 | 169 | ||
| 4.14 | 0,040 | ACTB protein | Homo Sapiens | 40536 | 5.55 | 15277503 | 12/57 | 45 | 93 |
| 4.02 | 0.050 | transthyretin variants | Homo Sapiens | 13806 | 5.57 | 2098255 | 10/42 | 81 | 153 |
| 3.59 | 0,049 | human manganese superoxide dismutase mutantQ143n (MnSOD) | Homo Sapiens | 22290 | 6.86 | 2780818 | 10/59 | 46 | 95 |
| 3.57 | 0,034 | glyceraldehyde-3 phosphate dehydrogenase | Homo Sapiens | 24776 | 8.68 | 89573929 | 8/39 | 47 | 89 |
| 3.32 | 0,021 | heat shock protein 27 | Homo Sapiens | 22427 | 7.83 | 662841 | 10/67 | 57 | 99 |
| 2.83 | 0,050 | mitochondrial aldehyde dehydrogenase | Homo Sapiens | 54394 | 5,6 | 6137677 | 17/71 | 43,3 | 134 |
| 2.76 | 0,037 | tubulin alpha -1C | Homo Sapiens | 50548 | 4.96 | 14389309 | 15/66 | 49 | 138 |
| 2.76 | 0,0084 | tubulin alpha | Homo Sapiens | 33321 | 5.86 | 37492 | 8/30 | 22 | 71 |
| 2.74 | 0,040 | glutamate carboxypeptidase | Homo Sapiens | 52700 | 5.7 | 15620780 | 14/40 | 38 | 143 |
| 2.57 | 0,024 | LAP 3 protein (leucyl aminopeptidase) | Homo Sapiens | 54724 | 6.80 | 37588925 | 15/46 | 38 | 149 |
* CyDye images were analyzed by BVA and spots that showed statistically significant differences (Student's t-test) in intensity between the control and the GBM groups are listed. (a) Average ratio GBM/NT: The average ratio value indicates the standardized volume ratio between the two groups: GBM and non tumorous patients. A two-fold increase is represented by 2. (b) T-test: Student's T-test p value: Only proteins with ratios showing significant differences (p£0.05) were retained. (c):Mt th: Theoritical relative mass. (d) PI th: Theoritical isoelectric point. (e) GeneBank sequence identification number(f): Number of matched peptides versus total number of peptides. (g): Percent coverage of the protein. (h) Mascot score: Protein scores greater than 81 are significant (p < 0.05).