. 2011 Apr 6;11:67. doi: 10.1186/1471-2180-11-67

Table 1.

E. faecalis strains and plasmids used in this study.

strain or plasmid	characterization	reference
strains

E. faecalis 12030	Clinical isolate, strong biofilm producer	[33]

E. faecalis ATCC 29212	Reference strain

E. faecalis 12030ΔbgsA	(EF2891) bsgA mutant	[5]

E. faecalis 12030ΔbgsB	bgsB deletion mutant	This study

E. faecalis 12030ΔbgsB_rec.	Reconstituted mutant	This study

Escherichia coli DH5α	Gram-negative cloning host

Escherichia coli TOP10	Gram-negative cloning host	Invitrogen



plasmids

pCASPER	Gram-positive, temperature-sensitive mutagenesis vector	[34]

pCRII-TOPO	Gram-negative cloning vector	Invitrogen

pCASPER/ΔbgsB		This study

pMAD/bsgB		This study

pMAD	oripE194^ts, Em^R, Amp^R, bgaB	[35]