Table 1.
Classification of TFS groups of genes responding to testosterone and/or MAA
| Class | Description | TFS group | TFS Gene Count | Response to | |||
|---|---|---|---|---|---|---|---|
| T | MAA | T/MAA vs. T | T/MAA vs. MAA | ||||
| I | No interaction between T and MAA | 9.1001 | 472 | Up | - | - | Up |
| 9.2002 | 492 | Down | - | - | Down | ||
| 6.0110 | 350 | - | Up | Up | - | ||
| 6.0220 | 174 | - | Down | Down | - | ||
| 3.1100 | 53 | Up | Up | - | - | ||
| 3.2200 | 55 | Down | Down | - | - | ||
| 15.2112 | 59 | Down | Up | Up | Down | ||
| IIa | MAA enhances action of T | 8.0001 | 421 | - | - | - | Up |
| 8.0002 | 398 | - | - | - | Down | ||
| 14.0111 | 60 | - | Up | Up | Up | ||
| 14.0222 | 24 | - | Down | Down | Down | ||
| IIb | T enhances action of MAA | 4.0010 | 355 | - | - | Up | - |
| 4.0020 | 264 | - | - | Down | - | ||
| 13.1011 | 144 | Up | - | Up | Up | ||
| 13.2022 | 56 | Down | - | Down | Down | ||
| IIc | T enhances action of MAA, and MAA enhances action of T | 15.1111 | 77 | Up | Up | Up | Up |
| 15.2222 | 39 | Down | Down | Down | Down | ||
| 12.0011 | 316 | - | - | Up | Up | ||
| 12.0022 | 81 | - | - | Down | Down | ||
| IIIa | MAA blocks action of T | 1.1000 | 329 | Up | - | - | - |
| 1.2000 | 307 | Down | - | - | - | ||
| IIIb | T blocks action of MAA | 2.0100 | 302 | - | Up | - | - |
| 2.0200 | 165 | - | Down | - | - | ||
| IIIc | MAA blocks the action of T, but MAA alone shows no activity | 5.1020 | 62 | Up | - | Down | - |
| 5.2010 | 52 | Down | - | Up | - | ||
| IIId | T blocks the action of MAA, but T alone shows no activity | 10.0102 | 107 | - | Up | - | Down |
| 10.0201 | 16 | - | Down | - | Up | ||
Regulated genes were clustered based on whether there is an interaction between testosterone and MAA, as follows: Class I represents genes that respond to testosterone or to MAA with no interactive effect. Class II represents genes whose response to testosterone was enhanced by MAA (IIa), genes whose response to MAA was enhanced by testosterone (IIb), and genes whose responses to testosterone and MAA showed mutual enhancement (IIc). Class III corresponds to genes whose response to testosterone could be blocked by MAA (IIIa, IIIc) or whose response to MAA could be blocked by testosterone (IIIb, IIId). See Additional file 2, Table S2C for further details.