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. 2011 Apr 15;12(1):48. doi: 10.1186/1465-9921-12-48

Table 2.

List of the antibodies, concentrations and antigen-retrieval methods used in the study.

Antibody Source Concentration Antigen retrieval
α-SMA Dako 1:1000 MW 19 min in tris-EDTA‡

Desmin Dako 1:300 MW 19 min in tris-EDTA

Tn-C Biohit 1:1000 MW 30 min in tris-EDTA

Vimentin Dako 1:1500 MW 14 min in citrate†

MW = microwave heat treatment; ‡ = tris/EDTA buffer, pH 9.0; † = citrate buffer, pH 6.0