Table 2.
Antibody | Source | Concentration | Antigen retrieval |
---|---|---|---|
α-SMA | Dako | 1:1000 | MW 19 min in tris-EDTA‡ |
Desmin | Dako | 1:300 | MW 19 min in tris-EDTA |
Tn-C | Biohit | 1:1000 | MW 30 min in tris-EDTA |
Vimentin | Dako | 1:1500 | MW 14 min in citrate† |
MW = microwave heat treatment; ‡ = tris/EDTA buffer, pH 9.0; † = citrate buffer, pH 6.0