Figure 1.

In vitro transcription of HEV genomic replicon. The BglII-linearized plasmid, pSK-GFP(replicon), (5 μg) was transcribed in a 50 μl reaction volume and cooled on ice. A 2.5 μl of the RNA mixture was checked for RNA integrity and semi-quantitation on a 1% agarose gel, containing ethidium bromide. DNA. mr.(standard DNA marker); Neg.cont.(negative control).