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mRNA levels of IR, IRS-1, IRS-2 and GLUT4. C: control diet fed animals. HF: high-fat diet fed animals. F: Fasted animals. NF: Non fasted animals. (A) Skeletal muscle (SM). (B) Retroperitoneal adipocytes (RP). (C) Subcutaneous adipocytes (SC). Data are means ± SEM of the ratio between each gene and GAPDH expression and are referred to gene expression in control-diet fed, fasted group (C-F = 100). Groups (F vs NF) were compared using the Mann-Whitney U test: T < 0.10; *p < 0.05.
