Figure 2. Inhibition rate of dichlorvos (A), chlorpyrifos-oxon (B) and carbofuran (C) in the presence or absence of BxACE-3.

For all of the tested inhibitors, the enzyme mixture without BxACE-3 (brown line) was the most inhibited, whereas the enzyme mixture with BxACE-3 pre-incubated for 10 min with inhibitors (green line) was the least inhibited. The enzyme mixture mixed at the same time (orange line) and the enzyme mixture with BSA pre-incubated for 10 min with inhibitors (yellow line) showed middle inhibition. The inhibition rates in response to chlorpyrifos-oxon, dichlorvos and carbofuran were statistically different between enzyme mixtures without BxACE-3 and with BxACE-3, as determined by GLM repeated measures ANOVA (p<0.05).