Figure 1. Flowchart of the sample preparation.

A) Steps a through e explain the main steps in Illumina sample preparation, a) the initial genomic DNA, b) fragmentation of genomic DNA, c) end repair, d) addition of A bases to the fragment ends and e) ligation of the adaptors to the fragments. B) Overview of the automated the size selection protocol presented here. The first precipitation discards fragments larger than the desired interval. The second precipitation selects all fragments larger than the lower boundary of the desired interval.