Table 1. Sequencing run information.
Lane | Sample | Note | Conc. (pM) | Clusterdensity | # seq. pairs | # seq. pairs PF | >Q30 (%) | PF (%) |
1 | Spruce | Manual | 6 | 400 | 73 768 362 | 66 246 185 | 89 | 90 |
2 | Spruce | Manual | 7 | 445 | 81 981 446 | 73 154 482 | 88 | 89 |
3 | Spruce | Manual | 8 | 488 | 89 947 283 | 79 179 058 | 87 | 88 |
4 | Spruce | Auto | 7 | 633 | 116 675 822 | 98 821 657 | 84 | 85 |
5 | U-2 OS | Auto | 7 | 644 | 118 762 987 | 99 614 234 | 85 | 84 |
6 | U-2 OS | Auto, Manual gel cut | 7 | 504 | 92 847 597 | 80 785 904 | 87 | 87 |
7 | Spruce | Auto, NEB | 7 | 653 | 120 322 872 | 101 014 779 | 84 | 84 |
8 | A-431 | Auto, NEB | 7 | 718 | 132 257 940 | 106 038 199 | 84 | 80 |
Input parameters and result statistics from clustering and sequencing performed on manually and automatically prepared DNA libraries from plant and human sources.