Figure 3.

Sigma-1 receptor antagonist BD1047 restores E-S potentiation in CIE-withdrawn hippocampal slices. Data are presented as described in Fig 2 legend. A, E-S coupling relationship before and at 60 min after 100 Hz stimulations for an individual CIE-withdrawn slice pretreated with BD1047 in the bath solution (1 μM; beginning at 30 min before and with continuous perfusion throughout the recording period). Boxed data points before (1) and after (2) tetanus stimulations are from the representative traces shown on the left. Note the leftward shift in the E-S curve after tetanization, consistent with E-S potentiation in this example. B, Frequency distribution of the E₅₀ shift at 60 min after tetanization in BD1047 pretreated naïve or CIE-withdrawn slices. Also shown are the fitted Gaussian distributions (solid and dashed curves: R² = 0.9655 and 0.9938, pretreated naïve and CIE-withdrawn, respectively; see Fig. 2E legend for details). C, Mean E₅₀ values at 60 min after LTP induction in naïve and CIE-withdrawn slices pretreated with BD1047 (1 μM; +BD1047). Data from slices not exposed to BD1047 (−BD1047 or ACSF only) are included from Fig. 2F for comparison. Mean values ± SEM are shown for the indicated number of slices. +++, p < 0.001 Naive versus CIE-withdrawn (no BD1047) and **, p < 0.01 CIE withdrawn −BD1047 versus +BD1047 by Bonferroni post t-test comparisons.