Table1.
Summary statistics from paired-pulse inhibition analyses in naïve control and CIE withdrawn hippocampal slices
| Drug | Tetanus | Group | N | Paired-pulse Inhibition Maximal strength |
N | Paired-pulse Inhibition Near half-maximal strength |
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|---|---|---|---|---|---|---|---|---|---|---|
| Baseline | 60 min | % change | Baseline | 60 min | % change | |||||
| ACSF | 100 Hz | Naive | 15 | 77.6 ± 6.4 | 55.1 ± 7.7 | −32.7 ± 6.8### | 8 | 71.9 ± 12.2 | 49.9 ± 10.4 | −46.2 ± 10.6# |
| CIE | 17 | 63.3 ± 5.6+ | 39.1 ± 8.4+ | −44.3 ± 9.6### | 7 | 72.3 ± 4.4 | 46.7 ± 7.1 | −44.1 ± 9.7# | ||
|
| ||||||||||
| ACSF | none | Naive | 9 | 72.1 ± 10.0 | 66.6 ± 9.1 | −3.0 ± 6.1* | ||||
| CIE | 10 | 74.5 ± 10.7 | 75.4 ± 10.1 | −7.6 ± 6.6* | ||||||
|
| ||||||||||
| D-AP5 | 100 Hz | Naive | 8 | 68.6 ± 11.5 | 63.8 ± 8.3 | −3.0 ± 4.5* | 8 | 84.3 ± 13.6 | 69.5 ± 11.7 | −4.8 ± 3.4* |
| CIE | 12 | 73.1 ± 13.3 | 70.5 ± 15.0 | −7.7 ± 4.6* | 9 | 69.7 ± 9.8 | 74.7 ± 8.2 | −3.1 ± 5.2* | ||
|
| ||||||||||
| 3α5β-S | 100 Hz | Naive | 9 | 76.0 ± 6.8 | 52.5 ± 12.2 | −46.5 ± 11.0# | 7 | 66.0 ± 7.7 | 52.1 ± 13.9 | −8.4 ± 13.4* |
| CIE | 7 | 82.4 ± 9.6 | 70.8 ± 5.2* | −26.8 ± 5.3# | 7 | 94.6 ± 3.5+ | 76.8 ± 10.2+* | −19.0 ± 9.8* | ||
|
| ||||||||||
| BD1047 | 100 Hz | Naive | 9 | 64.8 ± 9.9 | 50.1 ± 13.2 | −34.3 ±13.5# | ||||
| CIE | 10 | 64.9 ± 11.4 | 40.9 ± 12.3 | −52.8 ± 8.3### | ||||||
Mean percentage inhibition (2nd versus 1st pop. spike amplitude) values ± SEM at baseline and 60 min with or without 100 Hz tetanus stimulations in ethanol-naïve (Naïve) or CIE-withdrawn (CIE) hippocampal slices recorded under the conditions indicated (N number of slices). Data are from slices that exhibited at least ~30-40% initial paired-pulse (10 ms inter-pulse interval) inhibition during stability tests. Drugs indicated were added to the flow of the ACSF bath solution at 30 min prior to baseline and for the remaining duration of the recordings (3α5β3-S 5 μM; D-AP5 50 μM; BD1047 1 μM). % change indicates the decrease in paired pulse inhibition at 60 min relative to the baseline value using within-slice comparisons such that greater negative values indicate stronger disinhibition by LTP.
p < 0.05
p < 0.001 versus no change by a one-sample t-test.
p < 0.05 main effect of CIE treatment versus matched naïve control across both time points by 2-way ANOVA;
p < 0.05 versus matched ACSF (no drug), 100 Hz by a Bonferroni t-test comparison.