Table 2.
Antibiotic susceptibility assays
| Zone of inhibition (mm)a | |||||
|---|---|---|---|---|---|
| Strain | Aztreonam (30 µg) | Cefotaxime (300 µg) | Cinoxacin (100 µg) | Sulphonamides (300 µg) | Carbenicillin (100 µg) |
| PAK + pMMB67-rocS2 | 15.5 ± 0.7 | 8 ± 0.2 | 8.5 ± 0.8 | 4 ± 0.8 | ND |
| PAKΔrocA2 + pMMB67-rocS2 | 7.5 ± 0.4 | 3 ± 0.2 | Rb | R | ND |
| PAK + pMMB67-rocS1 | 15 ± 0.6 | 8 ± 0.1 | 7 ± 0.5 | 4 ± 0.7 | 13.5 ± 0.6 |
| PAKΔrocA2 + pMMB67-rocS1 | 7.5 ± 0.3 | 2.5 ± 0.1 | R | R | 5.5 ± 0.7 |
a
Zones of growth inhibition around discs were determined by the disc diffusion assay on M63 minimal media agar plates. The mean and standard deviation of a representative experiment with biological triplicates are given and the experiment has been repeated twice.
b
R, resistant, no growth inhibition observed.
All differences observed between PAK/pMMB67-rocS2 and PAKΔrocA2/pMMB67-rocS2 or PAK/pMMB67-rocS1 and PAKΔrocA2/pMMB67-rocS1 are significant with a P-value < 0.01.
ND, Not determined.