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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Behav Pharmacol. 2011 Jun;22(3):275–280. doi: 10.1097/FBP.0b013e328345f758

Figure 1. Behavioral effects of acute 20-min exposure to 3,4-Methylenedioxymethamphetamine (MDMA) on zebrafish tested in the standard 6-min novel tank test (Experiment 1; n = 27 (controls), 28 (10 mg/L), 12 (40 mg/L), 27 (80 mg/L) and 12 (120 mg/L).

Figure 1

Figure 1

A – Manually recorded behavioral endpoints (*P<0.05, *** P<0.001, # P=0.05–0.01 (trend) vs. control; post-hoc Tukey test for significant ANOVA data). B – Two-dimensional (2D) traces generated using Ethovision XT7 software and a side-view camera. 2D traces were examined for each experimental cohort, rated from 1 to n (based on similarity to each other), and the middle trace was selected as representative, to best illustrate the typical patterns of zebrafish locomotion. C – Habituation (per-minute distribution) of zebrafish behavioral activity (* P<0.05, *** P<0.001, min 1 vs. min 6 of the respective drug group, U-test).