Figure 2. Immediate behavioral effects of 40 and 80 mg/L MDMA on zebrafish tested in the 30-min novel tank containing drug-treated water (Experiment 2; n = 12 in each group).
ANOVA analyses revealed significant dose effect for time in top (F(2,35) = 11.2, P<0.0001) and freezing bouts (F(2,35) = 11.2, P<0.05) but not for latency to top (F(2,35) = 1.9, NS), transitions to top (F(2,35) = 0.9, NS), freezing duration (F(2,35) = 1.6, NS) or erratic movements (F(2,35) = 0.1, NS). Line diagrams with per-minute distribution of activity are presented only for two endpoints with significant dose effect. Post-hoc Tukey test for significant ANOVA data: for bar diagrams *P<0.05 vs. respective controls; for line diagrams *P<0.05, *** P<0.0001 vs. the respective minute’s control value. Note marked behavioral effects (increased time in top) starting to appear in zebrafish within the first 5–10 min of the drug treatment.