Figure 5.

Inhibitory effects of MK-2 on nuclear translocation of NF-κB in RAW264.7 cells. The cells were incubated with 10 μM MK-2 (+) or DMSO (−), as a vehicle control for 30 min, and then with 100 ng/mL LPS for 30 min. Nuclear proteins were prepared from the cells and subjected to Western blot analysis for evaluation of the nuclear translocation of NF-κB p65. The intensity of each band was analyzed, and the values relative to treatment without LPS (negative control, taken as 1.0-fold) are represented at the lower edge of the image.