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. 2011 Feb 22;12(2):1334–1358. doi: 10.3390/ijms12021334

Figure 1.

Figure 1.

Claudin 4 RNA and protein expression in ovarian cancer tissues and cell lines. (A) Claudin 4 RNA expression in 13 ovarian cancer cell lines and 7 NOSE cell lines as determined by qRT-PCR. Expression values shown as fold change over the lowest expressing cell line (1816–575), and are the average of two experiments (see Experimental section). (B) Claudin 4 protein expression was determined by Western immunoblot analysis of ovarian cancer and NOSE cell lines (50 μg protein/lane). β-actin serves as a loading control. (C) Claudin 4 protein expression was determined by Western immunoblot analysis of 7 primary stage III/IV serous ovarian cancer tissues (C1–C7) and 5 normal ovaries (N1–N5) (50 μg protein/lane). β-actin, loading control.