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. 2011 May;31(5):415–422. doi: 10.1089/jir.2010.0094

FIG. 2.

FIG. 2.

Kinase activity of IRAK2 is required to stabilize LPS-induced cytokine and chemokine mRNA. (A) IRAK2-deficient macrophages were restored by IRAK2-WT, IRAK2-KD, or empty vector, and put back cells were pretreated with LPS (1 μg/mL) for 90 min and then treated with actinomycin D (5 μg/mL) and LPS (1 μg/mL) for indicated times. KC and IL-6 mRNAs were analyzed by quantitative reverse transcription–polymerase chain reaction (PCR), normalized by β-actin, and plotted. Results shown are the mean and standard deviation of 3 independent experiments (P < 0.05). (B) IRAK2-deficient macrophages were restored by IRAK2-WT, IRAK2-KD, or empty vector. The put back cells were treated with LPS for indicated times and analyzed by Illumina microarray analysis. A heat map is shown for genes that are induced to similar levels in IRAK2-WT and IRAK2-KD cells at 0.5 h, but much lower in the IRAK2-KD than the IRAK2-WT cells at 4 h. (C) Confirmation of some genes shown in B with real-time PCR.