Skip to main content
. 2011 May;31(5):459–469. doi: 10.1089/jir.2010.0115

FIG. 5.

FIG. 5.

IL-10 does not mediate IL-27-induced IL-17 inhibition. Naïve CD4+ T cells were activated with CD3/CD28 in the absence and presence of Th17 polarizing cytokines with and without IL-27 in either serum-containing medium (left graph) or serum-free medium (right graph) and IL-10 levels were measured in supernatants after 6 days by ELISA (A). Data are expressed as the mean ± SEM of 14 different healthy individuals (left graph) and 8 different healthy donors (right graph). *P = 0.03. (B) ELISA of IL-10 in supernatants from naïve T cells activated as in (A) in 10% FCS/RPMI in the presence of anti-inducible T-cell co-stimulator (ICOS). Results are expressed as the mean ± SD of 3 different donors. (C) ELISA of IL-17, IFN-γ, and IL-10 in supernatants from naïve T cells activated as in (A) in serum-free conditions in the presence of IL-10 Abs. (D) Intracellular staining of cells cultured under Th17 polarizing conditions in the presence of IL-27 and anti-IL-10. One of 3 representative donors is shown.