Figure 1.

Timeline showing the temporal relationship between key events in the study and two seasons of Leishmania major transmission. Two hundred participants living in endemic areas of zoonotic cutaneous leishmaniasis (ZCL) in Central and Southwestern Tunisia (Gafsa and Sidi Bouzid) were followed up over 2 years throughout two seasons of L. major transmission. Several parameters such as leishmanin skin test or the presence of typical scars were monitored at the beginning of the study and after each transmission season and the triggering of new cases. Peripheral blood samples were obtained from each donor during these examinations.