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. 2011 Apr 18;121(5):1974–1985. doi: 10.1172/JCI44900

Figure 1. ECs express PlexinD1 in response to VEGF signaling in the developing retinal vasculature.

Figure 1

(A) Whole-mount ISH for Vegf in combination with IHC for type IV collagen (Col IV) in the P4 retina. Vegf is expressed in astrocytes ahead of the advancing vessels and around the preformed capillaries and veins, but not around arteries. (B) High-magnification image of the advancing vascular front. Vegf-expressing astrocytes (red) reside adjacent to the tip (arrowhead) and stalk (arrows) ECs (green). (C) ISH for Plxnd1 in combination with IHC for Col IV in the P4 retina. Plxnd1 is expressed in ECs of the developing retinal vasculature, except in arteries. (D) High-magnification image of the advancing vascular front. Plxnd1 (red) is expressed in the tip (arrowheads) and stalk (arrows) ECs (green). (E) ISH for Plxnd1 and IHC for Col IV in P4 retinas 8 hours after intravitreal protein injection. Endothelial Plxnd1 expression is downregulated by VEGFR1-Fc, whereas neuronal Plxnd1 expression is maintained in a dot-like pattern. Note the faint Plxnd1 expression in arteries in the control retina. A, artery; V, vein. Scale bars: 100 μm (A, C, and E); 20 μm (B and D).