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. 2011 Apr 11;121(5):1882–1893. doi: 10.1172/JCI42556

Figure 2. DKK2 protein induces angiogenesis in vivo.

Figure 2

(A and B) Matrigel plugs treated with VEGF (200 ng) and DKK2 (1 μg) were excised from mice 5 days after injection (n = 5 per group). Scale bars: 100 μm. CD31 staining (A) and quantification of hemoglobin concentrations in a Matrigel plug (B). White arrows indicate CD31-stained vessels. (C–F) Corneal angiogenic responses induced by VEGF-containing (200 ng) or DKK2-containing (1 μg) micropellets. Scale bars: 500 μm. Asterisks indicate micropellet inserted (C). 5 days after pellet implantation, Evans blue was injected i.v. After 30 minutes, each mouse eye was photographed. Scale bars: 200 μm (D). CD31 and NG-2 staining of cryosections of the eye (E). Scale bars: 100 μm. Green, CD31 positive; red, NG-2 positive; blue, DAPI. Pericyte coverage calculated as a ratio of the NG-2 to CD31 staining area (F). Data represent mean ± SD. ***P < 0.001.

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