Figure 5. DKK2 increases filopodial protrusions in a Cdc42-dependent manner.

(A) HUVECs stably expressing eGFP, DKK1, or DKK2 were analyzed for Cdc42 activities. (B) Stable transfectants were cultured on Matrigel-coated plate (morphogenesis) for 2 hours and Cdc42 activities were measured. (C and D) HUVECs were transiently transfected with expression plasmids encoding eGFP control or eGFP dominant negative mutant of Cdc42 (Cdc42 N17). After 24 hours, these cells were plated on Matrigel-coated plates and incubated with PBS or DKK2 (1.5 μg/ml) for 2 hours. Then microphotographs were taken (C) and filopodia number was quantified (D). Arrowheads indicate filopodial extension. Data represent mean ± SD. **P < 0.01. Scale bars: 20 μm. (E) HUVECs stably expressing eGFP or DKK2 were incubated with sFRP (200 ng/ml) for 24 hours and Cdc42 activity was measured.