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. 2011 Apr 25;121(5):1986–1999. doi: 10.1172/JCI43839

Figure 2. Lung microvascular endothelium does not internalize TNFR1.

Figure 2

Lung microvessels were sequentially infused with red fluorescent calcein red (5 μM, 20 minutes), then (A–C) green fluorescent FITC-conjugated albumin (5%, 10 minutes) or (D–F) green fluorescent sTNF-α–488 (1 ng/ml, 10 minutes). Each infusion was followed by buffer infusion for a further 20 minutes, then by TB. Scale bars: 25 μm. Colocalization of red and green fluorescence is indicated by yellow pseudocolor in the microvessel (B) and in the high-power image of the boxed region showing a single EC (C). Note the lack of colocalization for sTNF-α (F and G). *P < 0.05 versus calcein. n = 4 per group.