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. Author manuscript; available in PMC: 2011 Apr 28.
Published in final edited form as: Mol Biol Rep. 2009 Feb 26;37(3):1155–1163. doi: 10.1007/s11033-009-9476-8

Fig. 1.

Fig. 1

Amplification of the SUMO1 promoter region from human genomic DNA. a Sequence of the SUMO1 promoter region derived from GenBank. The location of three mapped cDNA 5′ ends (GenBank Accession numbers BC006462, BC066306, and BC053528) are indicated with arrows, and the most 5′ position was designated the +1 nucleotide. Predicted binding sites for the Whn transcription factor and for Sp1 are as indicated; numerous other potential transcription factor binding sites are located in the region, but at not shown for simplicity. The 5′ endpoints of the truncations used in Fig. 3 are indicated as T2, T3, and T4. b Agarose gel of the PCR reaction use to amplify the SUMO1 promoter region. Lane 1 shows a 100-bp ladder and lane 2 is a sample of the PCR reaction using human genomic DNA and the primers described in “Materials and methods”