Effect of signaling pathways inhibitors on IL-8 induced neutrophil adhesion to ICAM-1. A. PI3k inhibitor wortmannin (500 nM) and B. PKC inhibitor BIM (1µM) had no effect. C. PLC inhibitor U73122 (2 µM) completely blocked ICAM-1 dependent adhesion, while its inactive analog U73343 (2 µM), which was used as a control, had no effect. Experiments with BIM and PLC inhibitors were performed at 37°C, experiments with wortmannin were performed at room temperature. Ninety-five cells from six donors were analyzed for these experiments. Error bars represent standard error. Pair-wise t-tests at each time point show no statistically significant difference between wortmannin treated, BIM-treated or U73343-treated cells compared to control at the 95% confidence level, whereas the U73122-treated cells were statistically different at all time points (p < 0.01).