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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Cell Mol Bioeng. 2010 Jun 1;3(2):106–116. doi: 10.1007/s12195-009-0099-x

Figure 4.

Figure 4

Effect of IP3 inhibitors caffeine and 2APB on IL-8 induced calcium release and neutrophil binding to ICAM-1. A. Inhibition of calcium spark by caffeine. While cells pre-incubation with 5mM caffeine was partially effective, 10mM caffeine completely inhibited IL-8 induced calcium release. B. Timing of calcium release inhibition by 2-APB. 20 µM 2-APB had a partial effect on calcium release induced by IL-8, while pre-incubation of cells with 60 µM 2-APB for 12 minutes completely inhibited IL-8 induced calcium release. C. Inhibition of IL-8 induced neutrophils adhesion to ICAM-1 by 2-ABP (60 µM) and caffeine (10 mM). 51 cells from 5 donors were analyzed for this experiment. Error bars represent standard error. Beginning at75 s and thereafter, adhesion probability in the presence of 2APBor caffeine is significantly different from control (Student’s t-test, p < 0.001). D. Video micrograph showing phagocytic cap in the control cells and in the cells pretreated with 2-APB or caffeine 90 seconds after IL-8 bead attachment.