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. Author manuscript; available in PMC: 2011 Apr 28.
Published in final edited form as: Mol Biol Rep. 2010 Mar 11;37(8):3863–3868. doi: 10.1007/s11033-010-0042-1

Fig. 2.

Fig. 2

Binding of MYBBP1A with Ipr1 and eGFP-Ipr1. Mouse J774A.1 clone 21 cells were transduced with lentivirus containing the vector of pHAGE-CMV.Flag-MYBBP1A.UBC.dLNGFR.W. After stimulated with IFN-g and/or DOX, transduced cells were processed into cytoplasm and nuclei. Nuclear extracts were incubated with either anti-Flag antibody or IgG isotype-matched control, and then precipitated with Protein G Microbeads. Eluate from immunoprecipitation and 1:10 amount of nuclear extract (input) were separated on 10% SDS-PAGE and then immunoblotted using anti-Ipr1 specific rabbit antiserum