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. 2011 May 1;25(9):901–906. doi: 10.1101/gad.2045111

Figure 4.

Figure 4.

DNA-binding effect of HJURP. (A) Position of the nucleosomal DNA with one of the H3–H4 heterodimers (PDB code: 1KX5) aligned with the HJURP complex. The shaded area indicates the β-sheet and L1 regions that obstruct the potential path of nucleosomal DNA in a CENP-A-containing nucleosome. (B) HJURP prevents spontaneous DNA binding. (Lane 1) Free 208-bp α-satellite DNA probe. (Lane 2) Spontaneous binding of CENP-A–H4 to DNA. (Lanes 3–5) Increasing amount of GST-HJURP reduces binding to DNA. (Lanes 6, 10) GST-HJURP or GST alone do not bind DNA. (Lanes 7–9) GST does not affect DNA binding. An asterisk indicates the position of a protein–DNA complex formed with the addition of HJURP and the CENP-A–H4 complex. Please note that the intensity of this band is stronger at a 1:1 than at higher HJURP-to-CENP-A–H4 molar ratios.