Figure 5.

PI(3,5)P₂ and the Cyc8–Tup1 complex are required for nuclear localization of Cti6 in Gal medium. (A) Cti6 localized primarily in the nucleus in fab1Δ cells in Glu medium, but Cti6 began to accumulate in the cytoplasm upon Gal shift, and a significant portion of Cti6 was observed in the cytoplasm at 4 h after Gal shift. Tup1 localized primarily in the nucleus at 4 h after Gal shift. (B) Cti6 localized in the nucleus in tup1Δ cells in Glu medium, but began to accumulate in the cytoplasm upon Gal shift. Similarly, in cyc8Δ cells, Cti6 localized in the nucleus in Glu medium, but a significant portion of Cti6 localized in the cytoplasm in Gal medium. Also, a significant portion of Cti6 often localized in the cytoplasm of cyc8Δ cells in Glu medium.