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(A) Comparison of the benzisoxazole elimination activity of AlleyCat (circles) with cCaM-F92Q (diamonds), [Protein] = 16 μM, [Substrate] = 0.5 mM. (B) Dependence of the activity of AlleyCat on [Ca2+] at pH 6.9, solid line shows the simulated two-site binding isotherm with an average Kd of 10-5 M. (C) pH profile of activity of AlleyCat. (D) Chemical (guanidinium hydrochloride, GdmCl) denaturation profiles of AlleyCat (circles) and cCaM (triangles).
