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Dissociation constants (Kd) of the native peptide sequences measured with competitive binding. (A) Peptides with the RXXR motif [P4, P7, P8, and RPARPAR (CR)] show higher binding affinity for NRP-1 than those with the XXXR motif (P13). (B) Three rounds of selection in MiPS device revealed two peptide sequences (P4 and P7) exhibiting higher affinity and specificity in binding to cells expressing NRP-1 compared with the well known CR.
