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. 2011 Apr 11;108(17):7022–7027. doi: 10.1073/pnas.1016811108

Fig. 3.

Fig. 3.

Expression levels of WAVE proteins, Sra-1, and Nap1 in Abi1-KO cells. Western blot analysis of MEF cell lysates of Abi1-KO control (fl/fl) and Abi1-KO cell lines. WAVE1, -2, -3, Nap1, Sra-1, and Abi2, protein levels were evaluated with specific antibodies in total cellular lysates obtained from two clones [c1 (#3-6) and c2 (#3-11)] lacking Abi1 expression and from the parental line MEF #3. A significant decrease in protein levels of the WAVE-complex components Sra-1 and Nap1, WAVE1, and total WAVE1/2/3 and a significant (twofold) increase in WAVE3 and Abi2 levels were observed in KO cells vs. control. Levels of protein expression ± SEM were evaluated based on band intensities from several independent experiments (Center). Anti-Rac1 antibody (Rac1) was used as loading control. Abi1 was detected with monoclonal antibody 1B9 (SI Materials and Methods).