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Near-UV CD-monitored titrations of metals to apo/metallated Atox1 (pH 6, 20 °C): (A) 50 μM apo-Atox1 and 50 μM Cu-Atox1 (1∶1); (B) CisPt (up to fivefold excess as indicated) added to 50 μM Cu-Atox1; (C) CisPt (up to fivefold excess as indicated) added to 50 μM apo-Atox1; (D) Cu (up to fivefold excess as indicated) added to 50 μM cisPt-Atox1 (1∶1).
