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Dysgenic (MDG) myotubes do not express the α1S subunit of the DHPR (CaV1.1). The expression of CaV1.1 was assessed by immunostaining in WT, dysgenic (MDG), and dysgenic (MDG)+CaV1.1 myotubes. Clusters of DHPRs (red foci) are located at the cell surface in WT and in dysgenic+CaV1.1 but not in dysgenic myotubes. Western blot confirms lack of CaV1.1 expression in dysgenic myotubes and its rescue by stable transduction. (Scale bar, 10 μm.)
