Fig. 6.

Increased [Ca²⁺]_rest in dysgenic myotubes does not correlate with frequency of spontaneous Ca²⁺sparks. Spontaneous LCREs were measured as described in Materials and Methods on WT and dysgenic (MDG) myotubes. The frequency of spontaneous LCRE was calculated for each individual cell, and the data are shown as mean ± SEM (Left; WT n = 10, dysgenic n = 10). In addition, the global fluorescence of each myotube was calculated and plotted (Right; WT n = 10, dysgenic n = 10) as mean ± SEM. ^#P < 0.05 (ANOVA). The effect of FK506 treatment on both LCRE frequency and global fluorescence is shown.