Fig. 4.

ATP-dependent ³H-l-fucose phosphorylation and GTP-dependent ³H-l-fucose activation. Whole-cell lysates (100 μg) of the indicated strains grown in the presence or absence of [³H]-l-fucose (as indicated) for 24 h and assayed for their potential to fucose under the indicated assay conditions. Ec, E. coli K12 wild-type; ΔEcfucK, E. coli K12 fucK mutant; Cj, C. jejuni NCTC11168 wild-type; Δcj0486, C. jejuni NCTC11168 Δ0486; Bt, B. thetaiotaomicron wild-type. The presence or absence of ATP and GTP are indicated by (+) and (−). Formation of modified ³H-l-fucose was found to be linear over the first 10 min of the assay. SDs are displayed by error bars. ³H-l-fucose alone did not show any significant binding to the column material.