Fig. 6.

BHK-21 cells were infected with a recombinant LCM virus expressing GFP, called r3LCMV-GFP at a multiplicity of infection of 0.1 in the presence of the indicated compound (50 μM). At 36 h postinfection, cells were fixed in 4% paraformaldehyde/PBS, and viral load was assessed based on GFP expression. Compound-associated cell (BHK-21) toxicity was determined by trypan blue. Compounds exhibiting cell toxicity of ≥30% (at 50 μM) were not evaluated for their antiviral activity. The figure shows examples of compounds with high (1 and 20), medium (35 and 33), low (41 and 34), or negligible (2 and 51) anti-LCMV activity. VC, treatment with vehicle (DMSO) (for further examples of compounds tested, see Table S1).