Fig. 3.
The hydrolysis of ATP during clathrin cage disassembly. The amount of Pi produced from ATP hydrolysis by Hsc70 during clathrin cage disassembly was monitored. Clathrin (0.33 μM triskelia), 0.35 μM auxilin, and 8 μM Hsc70 were mixed together. The reaction was initiated by addition of ATP (50 μM), and at specific time points samples were removed, quenched, and assayed for Pi content with malachite green solution. The data are fitted to a single exponential superimposed on a linear function (Eq. S2).