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Generation of Fstl1−/− mice. (A) Western blot analysis of Fstl1 proteins from E15.5 embryos (Upper) or E18.5 lung tissues (Lower). (B) Examination of neonates after birth revealed that Fstl1−/− neonates were cyanotic. (C) Autopsy observation showed that WT lungs were expanded by inhalation of air, which can be seen as air bubbles in the distal regions, but Fstl1−/− lungs were collapsed and did not show evidence of air in the distal airways (tr, trachea).
