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. 2011 Apr 28;7(4):e1001381. doi: 10.1371/journal.pgen.1001381

Figure 1. Impaired DNA damage response in Rnf168 deficient cells.

Figure 1

(A) Cells were subjected to IR or UV, and the extent of cell death was determined 24 hours later. Three independent experiments were performed. Data are presented as the mean ± SEM. *p<0.05. (B) Primary MEFs (passage 2) either untreated or irradiated (2 Gy) were stained with anti-phospho-histone H3 (pHH3) antibody and PI at 1 hour post IR. The percentage of mitotic cells was determined by FACS. Three independent experiments were performed. Data are presented as the mean ± SEM. *p<0.05. (C) Cell cycle progression post IR (10 Gy) treatment of WT and Rnf168−/− primary MEFs (Passage 2) was examined using BrdU/PI assay and FACS. Representative data are shown for three independent experiments. (D) Accumulation at the G2 phase of WT and Rnf168−/− primary MEFs (passage 2) post IR (10 Gy). Cell cycle profiles were examined by PI staining and representative data are shown from three independent experiments.