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. 2011 Apr 28;7(4):e1001381. doi: 10.1371/journal.pgen.1001381

Figure 4. Impaired long-term spermatogenesis in Rnf168−/− mice.

Figure 4

(A) Average litter sizes from intercrosses of 8-week-old mice with the indicated genotypes are shown [WT (F)×WT (M); n = 4, Het (F)×Het (M); n = 5, KO (F)×WT (M); n = 5, WT (F)×KO (M); n = 4, KO (F)×KO (M); n = 3. F = female, M = male, Het = Heterozygote (Rnf168+/−), KO = knockout (Rnf168−/−)]. (B) Twelve-month-old WT or Rnf168−/− males were mated with 8-week-old females. Number of pups from breeding 4 pairs of each genotype is shown. (C) Comparison of testes size in 8-week-old and 12-month-old WT or Rnf168−/− males. Bar, 3 mm. (D) Sections of testes stained with hematoxylin-eosin (left panels). Arrowheads indicate Leydig cells. Bar, 100 µm. The numbers of empty tubules per 100 seminiferous tubules are graphed (right panel). Data are presented as the mean ± SEM (WT n = 3; Rnf168−/− n = 3). *p<0.05. (E) Sections of epididymis stained with hematoxylin-eosin. Bar, 100 µm. Three mice of each genotype were analyzed.