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. 2011 Apr 28;7(4):e1002058. doi: 10.1371/journal.pgen.1002058

Figure 2. CHI–based screening identifies synthetic genetic interactions with CBK1 during morphogenesis.

Figure 2

(A) Examples of primary screening data for complex heterozygotes showing synthetic genetic interactions with CBK1; each strain was spotted on SM and incubated at 37°C for 3 days. Mutants with decreased peripheral invasion and decreased central wrinkling were selected. Representative positive scoring mutants from the primary screen are shown. An example of a strain complemented by re-integration of plasmid-borne CBK1 is shown. (B) Representative examples of independently constructed complex heterozygote strains showing complex haploinsufficient genetic interactions with cbk1Δ. (C) The ratio of pseudohyphal∶hyphal cells for the indicated strains was determined by light microscopy after 3 hours incubation in liquid SM at 37°C. The bars indicate mean values of two-three independent replicates of at least 100 cells. Error bars indicate standard deviation. Brackets indicate the results of Student's t test evaluation of differences between the indicated mutants; p<0.05 indicates a statistically significant difference. (D) Micrograph of filaments isolated from colonies of the parental cbk1Δ/CBK1 strain and the complex heterozygote cbk1Δ/CBK1 snz1Δ/SNZ1. Arrowheads indicate areas of hyphal-like morphology in the cbk1Δ/CBK1 mutant and pseudohyphae-like morphology in the cbk1Δ/CBK1 snz1Δ/SNZ1 double mutant.